A PNGase F digestion is one of the experimental procedures used. Briefly explain why this procedure would be used.

PNGase F is an active substance that provides catalysis of internal bond cleavage in oligosaccharides. Consequently, PNGase F can determine the modified ZNT1 chain, which was applied in the study. In particular, treatment of the cell lysate with catalysts allowed the band to be shifted from 75 kDa to 63 kDa, indicating that part of the molecule was detached.

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